Anticancer Ingaruka ya Ganoderma lucidum kuri selile ya Osteosarcoma

Ubushakashatsi bwacu bwerekana ko Ganoderma lucidum / reishi / lingzhi yerekana imiti igabanya ubukana bwa osteosarcoma muri vitro.Byagaragaye ko Ganoderma lucidum ibuza gukura kwa kanseri yamabere no kwimuka muguhagarika ibimenyetso bya Wnt / β-catenin.Irwanya kanseri y'ibihaha binyuze mu guhagarika icyerekezo no kwinjiza MDM2-yunganiwe na Slug degradation.Ganoderma lucidum irinda kanseri yamabere ikoresheje kugabanya inzira ya PI3K / AKT / mTOR, Ganoderma lucidum igira uruhare runini muri selile ikaze ya leukemia ihagarika inzira ya MAPK.

CCK-8 hamwe na koloni yibumbiye hamwe, kugirango isuzume ingaruka za Ganoderma lucidum kuri osteosarcoma selile selile viabilite no gukwirakwira, yerekanaga ko Ganoderma lucidum ihagarika ikwirakwizwa rya selile MG63 na U2-OS muburyo- kandi bushingiye kubitekerezo. ubushobozi bwa selile zo gukoroniza.

Ganoderma lucidum igenga imiterere ya genapapoptotique, kandi isesengura rya cytometrie ryerekanye ko apoptose ya selile MG63 na U2-OS yiyongera nyuma yo kuvurwa na Ganoderma lucidum.Kwimuka kwakagari nishingiro ryimyitwarire itandukanye yibinyabuzima, harimo angiogenezi, gukira ibikomere, gutwika, hamwe na kanseri metastasis.Ganoderma lucidum igabanya kwimuka no gutera imirongo yombi kandi ikabuza ikwirakwizwa, kwimuka, no gutera, kandi itera apoptose ya selile osteosarcoma.

Ibimenyetso bya Aberrant Wnt / β-catenin bifitanye isano rya bugufi no gushiraho, metastasis, na apoptose yubwoko butandukanye bwa kanseri, hamwe no kugenzura ibimenyetso bya Wnt / β-catenin bigaragara muri osteosarcoma.

Muri ubu bushakashatsi, umunyamakuru wa luciferase avuga ko Ganoderma ivura lucidum ibuza CHIR-99021 ikora Wnt / β-catenin.Ibi biragaragazwa kandi n’imyiyerekano yacu ko kwandukura ingirabuzimafatizo za Wnt, nka LRP5, β-catenin, cyclin D1, na MMP-9, birabujijwe iyo selile osteosarcoma ivuwe na Ganoderma lucidum.

Ubushakashatsi bwibanze bwerekanye mubyitegererezo byubuvuzi ko LRP5 itunganijwe muri osteosarcoma ugereranije nuduce dusanzwe, kandi imvugo ya LRP5 ifitanye isano nindwara ziterwa na metastatike no kubaho nabi kwindwara, bigatuma LRP5 ishobora kuvura osteosarcoma.

cat-catenin ubwayo nintego yingenzi muri Wnt / β-catenin yerekana inzira, kandi imvugo ya cat-catenin muri osteosarcoma iriyongera cyane.Iyo β-catenin ihinduye muri nucleus kuva cytoplazme, itangiza imvugo ya genes yayo yo hepfo, irimo cyclin D1, C-Myc, na MMPs.

Myc nimwe mubintu byingenzi bya proto-oncogène kandi bigira uruhare runini muguhuza imikorere, kwanduza, no kubuza imvugo ya gene.Byavuzwe ko guhagarika C-Myc oncogene itera gusaza na apoptose yubwoko butandukanye bwibibyimba, harimo osteosarcoma.

Cyclin D1 ningirabuzimafatizo yingenzi ya G1 igenzura kandi yihutisha inzibacyuho ya G1 / S.Kugaragaza cyane cyclin D1 birashobora kugabanya ingirabuzimafatizo no guteza imbere ikwirakwizwa ryihuse ryubwoko butandukanye bwibibyimba.

MMP-2 na MMP-9 ni stromelysine ifite ubushobozi bwo gutesha agaciro matrice idasanzwe, ikintu cyingenzi kubibyimba bya angiogenezi no gutera.

Ibi birerekana ko intego ya Wnt / β-catenin igira uruhare runini mugutezimbere kwa osteosarcoma, kandi ko guhagarika ibyo bimenyetso bishobora kugira ingaruka zikomeye zo kuvura.

Icyakurikiyeho, twabonye imvugo ya mRNA na proteyine ya Wnt / β-catenin yerekana ibimenyetso bifitanye isano na gen na PCR hamwe na blotting y'iburengerazuba.Muri iyo mirongo yombi, Ganoderma lucidum yabujije imvugo za poroteyine na gen.Ibisubizo birerekana kandi ko Ganoderma lucidum ibuza Wnt / β-catenin ibimenyetso byerekana LRP5, β-catenin, C-Myc, cyclin D1, MMP-2, na MMP-9.

E-cadherin ni transembrane glycoproteine ​​igaragara cyane muri selile epithelia kandi igahuza guhuza ingirabuzimafatizo na selile.Gusiba cyangwa gutakaza imvugo ya E-cadherin biganisha ku gutakaza cyangwa gucogora kwifata hagati ya selile yibibyimba, bigatuma selile yibibyimba bigenda byoroshye, hanyuma bigatuma ikibyimba cyinjira, kigakwirakwira, hamwe na metastasize.Muri ubu bushakashatsi, twasanze Ganoderma lucidum ishobora kugenzura E-cadherine, bityo ikarwanya Wnt / β-catenin-yunganiwe na selile ya osteosarcoma.

Mu gusoza, ibisubizo byacu byerekana ko Ganoderma lucidum ihagarika osteosarcoma Wnt / β-catenin yerekana kandi amaherezo igabanya ibikorwa bya selile osteosarcoma.Ubu bushakashatsi bwerekana ko Ganoderma lucidum ishobora kuba ingirakamaro kandi nziza yo kuvura osteosarcoma, ibicuruzwa bifitanye isano harimoganoderma lucidum spore amavuta softgels / reishi spore oil softgels,ganoderma lucidum spore ifu / ifu ya reishi